Training course about PCR work flow, which include
theory del about PCR and instrument of PCR and practical del (DNA extraction for blood sample to detect gene ARG1 233bp which responsible of asthma disease , PCR amplification for gDNA samples and agarose gel electrophoresis to run DNA amplified ) by molecular biologist assis.prof Ashti Said.
Title : Polymerase Chain Reaction Workflow
Summary: In this training course we will explain theory and practically ( Polymerase Chain Reaction workflow ) step by step from DNA extraction by kit and how will amplify this DNA and how prepare the agarose gel and will use.
Type of activity: Training Course Presenter:
Aza Bahadeen Taha
Ashti Mohammad Amin Said
Venue: Seminar Hall, Medical Research Center , HMU
No. participants: 20
Day: 8. Jan . 2022 to 12. Jan . 2022
Agenda Title of Agenda: Date Time Topic Presenter
|
Date |
Time |
Topic |
Presenter |
|
First day |
|||
|
8. Jan .2023 Sunday |
9:30 -1:00 |
Introduction about PCR and lab instrument |
Ashti M. Said |
|
Second day |
|||
|
9. Jan .2023 Monday |
9:30 -1:00 |
DNA Extraction |
Ashti M. Said |
|
Third day |
|||
|
10. Jan .2023 Tuesday |
9:30 -1:00 |
PCR Amplification |
Ashti M. Said |
|
Fourth day |
|||
|
11. Jan .2023 Wednesday |
9:30 -1:00 |
Gel Electrophoresis |
Ashti M. Said |
|
Fifth day |
|||
|
12. Jan .2023 Thursday |
9:30 -1:00 |
Discussion and question |
Ashti M. Said |
Iraq, Kurdistan Region,