Detection of High Risk Human Papillomavirus Types Infection in External Genital Warts from Females -Histopathological Study with PCR

Proceedings of the Third International Conference of the College of Medicine, Hawler Medical University

Detection of High Risk Human Papillomavirus Types Infection in External Genital Warts from Females -Histopathological Study with PCR

Shatha Zakar Said

Department of Pathology, College of Medicine, Hawler Medical University, Erbil, Iraq

DOI: 10.15218/com.03.06

Published: 21 Jan 2019

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Abstract

Background and objectives: External genital warts are relatively common sexually transmitted diseases among young women worldwide. There are as many as 170 HPV genotypes have been identified, and approximately 40 kinds of these types specially infect the anogenital tract. In the majority of cases, low-risk human papilloma-viruses (HPV), such as HPV-6 and HPV-11, are responsible but, high-risk types may also be detected and this has a bearing on vaccines for cervical and Vulvar cancer prevention. This study aimed to detect the high-risk HPV types like 16 and 18 in External genital warts from females in Erbil/ Iraq from 2010 – 2017.

Methods: Eighty five tissue samples of External genital warts from females in Erbil/Iraq involved in this study. Quantitative real-time PCR with gene specific primers and probes for the E6 gene of HPV-16, 18, and others, were used for the detection of HPV DNA in the tissue samples. After that, processing of the remaining tissue was done; H/E stain was used and examined microscopically.

Results: Out of 85 tissue samples of genital warts, 46 (54.1%) cases showed positive results for HPV infection by using PCR.  High risk group were detected in 5 of the 46 specimens (10.8 %), and 3 of them were positive only for HPV-16, one was positive for HPV-18 and other for HPV-33. None of these external genital warts showed any carcinomatous changes

Conclusion: In our society like Iraq, still the incidence of infection by high risk genotype HPV was low and none of them showed any carcinomatous changes, the use of vaccine for HPV infection still controversy.  However, additional studies with a larger population are required to prove the finding and help to determine the most appropriate genotype of HPV infected Iraqi women.

Keywords: High-risk HPV, HPV-16, 18 – PCR, External genital warts, Iraq.

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